Resources and assistance for Laboratories
Outreach is a critical component of the Laboratory Certification and Registration Program. We have compiled a list of forms, benchsheets, guidance documents, method references and training presentations to assist you in generating quality analytical results.
Welcome to the "Ultimate" BOD Resource. This web-based application is the culmination of eight years of training in both Wisconsin and Minnesota. To begin, simply click on a major subject button below or from the links at the side.
- About BOD
- A brief history of BOD including its origin and alternatives.
- Sample handling
- Sample handling information including holding time, preservation and storage information.
- Sample pre-treatment
- Information about the required preliminary testing associated with BOD.
- Equipment & supplies
- A look at the supplies and materials associated with BOD testing.
- DO probe options
- A look at DO probes and recent advancements.
- Calibrating a DO meter
- Perhaps the most important aspect of BOD testing: how to properly calibrate the DO meter and ensure acceptable blanks and QC.
- Pressure adjustments & calibration
- More detail regarding calibration. Specifically deals with how barometer pressure relates to calibration.
- Method details
- Information on specific aspects of BOD testing and method details including sample measurement and reporting.
- BOD dilution water
- Having good quality dilution water is critical for BOD testing. This section tells you how.
- Seed & seeding issues
- A detailed look at the importance of seed organism to BOD testing. Best sources of seed, how much to use and seed correction factor information.
- Nitrifiers, nitrification and cBOD
- A discussion of nitrification and the need to perform cBOD testing.
- BOD sample toxicity/sliding BODs
- How to determine if you have a toxic sample or if it looks like toxicity, but is something else.
- BOD quality control
- An overall summary of QA/QC requirements for BOD testing. Covers blanks, GGA, seed controls, replicates, control limits, and outlier testing.
- Troubleshooting BOD results
- Typical problems encountered in BOD testing and how to solve them.
- Other BOD resources
- A list of available books, websites and tools to guide you in your BOD testing.
Copyright 2006. University of Wisconsin Board of Regents.
Unauthorized use prohibited without the expressed written consent of the UW, State Laboratory of Hygiene and the DNR Laboratory Certification & Registration Program.
Many frequently requested documents are available for downloading.
The focus of these checklists is to cover procedures or requirements that are specifically mandated by code or method. They do not cover good lab practices and may not cover every single requirement. It remains the lab's responsibility to ensure that all proper procedures are followed. However, labs may find that the checklists are a good self-auditing tool to make sure there are not any major requirements being overlooked. Your auditor will review these requirements at an evaluation, but they will also go beyond these lists to provide required changes and procedural recommendations that should improve your lab results.
Download a checklist from the following table.
|BOD & cBOD checklist|
|Total phosphorus checklist|
|WWTP QC checklist|
|Full WWTP checklist Set|
These are benchsheets we have developed to assist your lab in compliance with NR 149 requirements. Benchsheets have been designed to address all critical method and code requirements for documentation and traceability.
Please note that benchsheets do change over time. Benchsheets are one of those things that can always be improved upon, so check this page frequently for updates. If the page has been updated in any way, the "Revised Date" at the bottom of the page will change.
Download a benchsheet from the following table.
Other QC-related spreadsheets/forms
Download a benchsheet from the following table.
|Pipet quarterly calibration worksheet|
This page is split into three parts: (1) actual methods that are somewhat unique to the state of Wisconsin; (2) some supporting documents that are helpful; and (3) a list of excellent web resources to help you locate methods.
Methods support information
Download method support information from the following table.
Web methods resources
The links below will take you to the web sites maintained by the U.S. Environmental Protection Agency. The department does not endorse the ideas, organizations or products listed at these sites. These sites are presented as a tool to locate information on the Web.
- National Environmental Methods Index (NEMI)
- EPA and USGS online database of methods and procedures for both regulatory and non-regulatory monitoring purposes for water, sediment, air and tissues.
- Manual For The Certification Of Laboratories Analyzing Drinking Water
- Fifth Edition PDF (209pp, 4M) EPA 815-R-05-004, January 2005
- Test Methods for Evaluating Solid Waste Physical/Chemical Methods
- SW-846, United States Environmental Protection, Office of Solid Waste, including updates and new methods.
- EPA CWA Analytical Test Methods Home
- The Methods collection contains all approved and other unapproved test methods for Clean Water Act testing. Also includes alternative test procedure approval and validation procedures.
- Recently Developed Methods from the EPA Office of Research and Development (ORD)
- EPA Safe Drinking Water Act methods 200.5, 321.8, 332.0, 415.3, 445, 521, 528, 529, 535, 549.2, and 556.
- National Service Center for Environmental Publications (NSCEP)
- This site was designed to provide easy access to EPA's publications and simple navigation to other services which may aide you in locating environmental information.
- EPA Manual of Manuals
- This document contains the titles, publication numbers, dates of publication, ordering information, abstracts, tables of contents, analyte-method cross reference lists, and Introductions to eight laboratory analytical chemistry methods manuals published between 1988 and 1995.
- DNR Biomonitoring Manuals and information.
- The State of Wisconsin Aquatic Life Toxicity Testing Methods Manual and other information on the Departments Biomonitoring Program.
- Environmental Monitoring and Assessment Program Surface Waters: Field Operations Manual for Lakes
- EPA/620/R-97/001. U.S. Environmental Protection Agency, Washington D.C., June 1997.
- Fish Sampling and Analysis
- The purpose of this manual is to provide overall guidance to states on methods for sampling and analyzing contaminants in fish and shellfish tissue that will promote consistency in the data they use to determine the need for fish consumption advisories.
- Evaluation of Dredged Material, Proposed for Discharge in Waters of the U.S.
- Testing Manual, Inland Testing Manual, United States Environmental Protection Agency, Office of Water (4305) Department of the Army, US Army Corps of Engineers, EPA 823-B-98-004, February 1998.
- EPA Whole Effluent Toxicity (WET Methods)
- Methods are guidelines establishing test procedures for data gathering and compliance monitoring under National Pollution Discharge Elimination System (NPDES) permits.
- Lake Michigan Mass Balance Study Methods
- Describes the sampling and analytical methods used in the Lake Michigan Mass Balance Study Methods Compendium. The compendium is organized into three volumes that describe sampling procedures used in the study and analytical procedures used by the Principal Investigators.
- Monitoring, Sampling, and Analysis, Office of Underground Storage Tanks, EPA
- A crucial part of cleanup actions is accurately and carefully measuring progress toward cleanup levels by monitoring sites, taking samples and analyzing these data to measure progress toward that goal.
- Index to EPA Test Methods, EPA
- Index to EPA Test Methods, 2003 edition, is also available in Portable Document Format (PDF).
- Links to Sources of EPA Test Methods on the Internet, EPA Region I
- Links to various EPA methods including air methods, lead in paint, monitoring well sampling, etc.
- Analytical Methods Developed by the Office of Ground Water and Drinking Water
- EPA Safe Drinking Water Act methods 300.1, 314.0, 314.1, 317.0, 326.0, 327.0, 331.0, 515.3, 515.4, 526, 527, 531.2, 532, 552.3, 556, and 556.1.
- Statement of Work, Contract Laboratory Program, Superfund, EPA
- CLP analytical services are defined in statements of work (SOWs). Statements of work provide the technical and contractual framework for commercial environmental testing laboratories to apply EPA CLP analytical methods for the preparation/isolation, detection, and quantitative measurement of organic target compounds and inorganic target analytes in water and soil/sediment environmental samples.
- EPA's Field Analytic Technologies Encyclopedia (FATE)
- This online encyclopedia is intended to provide information about technologies that can be used in the field to characterize contaminated soil and ground water, monitor the progress of remedial efforts, and for confirmation sampling and analysis for site close out.
General guidance documents
Guide to 2008 NR149 revisions
The following information can and should be used to help you develop procedures required by changes to NR 149 or to provide you with basic information regarding the structure and operation of the Laboratory Certification Program.
Information in this guidance will be updated over time. Please make sure to revisit this page and check to make sure you have the latest published version.NR 149 Summary Web Applet
The web page link above will pop open a new window that provides a cursory glance of major sections of the laboratory certification administrative rule (NR 149). Clicking on a link will pop up a smaller window which explains the rule in greater detail.
Quality manual template (4th Ed., June 2008)
The example Wastewater Treatment Plant Laboratory Quality Manual is designed to provide an example of the minimum documentation needed to fulfill the requirements of NR 149.37. This manual is not designed to be a complete guidance document for commercial laboratories, as it is written based on the analytical testing requirements associated with small wastewater treatment plants only.
Training & workshop materials
Beginning in 1998, the Laboratory Certification program has been involved with developing and presenting training sessions on a wide array of subjects. You will find, below, links to a PDF copy of handouts from these sessions. The handouts match the PowerPoint slides from the sessions.
- Ammonia & Phosphorus
- Back to Basics [(General Chemistry, Lab Practices)]
- Chemical Additions
- Chlorine & Disinfection
- Distance Learning
- Hexavalent Chromium
- ICP & ICP/MS
- Lab Math
- Mercury, Ultra-Trace
- On-Site Evaluation Process
- Operator Certification Exams
- Pipettor Calibration
- Reagent Water Requirements
- Reporting Requirements
Either browse through all the tables below or use the links above to jump directly to a portion of the table that interests you.Ammonia & Phosphorus Balances Back To Basics (General Chemistry, Lab Practices) Biochemical Oxygen Demand (BOD) Calibration Considerations Chemical Additions Monitoring for Water Supplies Chlorine & Disinfection Distance Learning
|Technology for Creating Utility Training
Technology can be used to produce on-line (distance)training for water utility operators. Training can be designed to meet needs of water utilities and regulators.
|ICP - You CAN'T Just Push Play (color)
This session was devoted entirely to interference correction. including IECs, FACT, and MSF. A whole new PT challenge sample was constructed, as well as a "Real World" sample designed to show the need for internal standard adjustment when faced with a "matrix". Screen scans explain the reason(s) why labs had trouble with specific elements. 5.3Mb. Download this for viewing on a PC screen.
|ICP - You CAN'T Just Push Play (black&white)
This session was devoted entirely to interference correction. including IECs, FACT, and MSF. A whole new PT challenge sample was constructed, as well as a "Real World" sample designed to show the need for internal standard adjustment when faced with a "matrix". Screen scans explain the reason(s) why labs had trouble with specific elements. 6.4Mb. Download this for printing to minimize use of toner.
|ICP - Secrets for Determining IECs
A shorter presentation focusing on the art of proper determination of interelement correction (IEC) factors for successful ICP analysis.
|Advanced ICP Tips & Techniques
This follow-up to the initial ICP training session focuses even more intently on interferences and background correction. The highlight of the session was the analysis, by participating labs, of a highly challenging PT sample designed by Wibby Environmental. Obtaining accurate results on "the Wibby" required a lab to be acutely aware of the myriad of interference problems associated with ICP technology.
|ICP from Ar to Zn
Excellent 2 day session covering basic aspects of ICP technology including theory, quality control, and a detailed section on interference identification and correction.
|Basic Principles of ICP/MS
Part of a 2-day ICP training session. The materials focus on providing an overview of ICP/MS technology including how the instrument operates.
|Low Level Mercury - Importance of Clean Sampling
Provides an overview of mercury contamination in the environment, closing with a detailed look at the "Clean Hands - Dirty Hands" technique for sample collection.