Wisconsin Administrative Code ch. NR 499 was created as authorized by ss. 285.51, Wis. Stats., which establishes a program for the department to develop a program of certification for persons participating in, or responsible for, the operation of…
Outreach is a critical component of the Laboratory Certification and Registration Program. We have compiled a list of forms, benchsheets, guidance documents, method references and training presentations to assist you in generating quality analytical…
Sample toxicity Often referred to as "sliding" BODs BOD decreases as sample volume increases (i.e., less dilute) Occurs frequently in systems receiving industrial waste Amounts to killing off (or severe shock to) "the bugs" Results in under-…
Seed preparation and addition De-ionized water kills - Adding a seed capsule to deionized water causes an osmotic imbalance that will kill seed organisms. The purified water —containing no salts— rushes across the bacterial cell walls. The bacteria…
Water and wastewater samples are biologically active, and the BOD will quickly deteriorate. Minimize the time between sampling and analysis to obtain the best BOD results. Take the following precautions when sampling and handling BOD samples.…
Websites Field Training Manual for Laboratory Analysts: Chapter 7 - BOD Biochemical Oxygen Demand Source: http://home.windstream.net/mikeric/Chap1to9/Chap7%20Biochemical%20Oxygen%20Demand.htm-->
Limit of detection (LOD) BOD detection limits are theoretically based. A key assumption which the concept of detection for BOD is based is that the LEAST amount of depletion allowable is 2 mg/L. The LOD is also based on the highest volume of sample…
Because the BOD test is a bioassay, it is critical to maintain optimal conditions for the "bugs" (bacteria and other microorganisms) to remain viable. Temperature, pH, oxygen levels and the presence of disinfectants can all influence the outcome of…
The following are the critical steps in preparing and analyzing samples for BOD testing. The BOD test is a method-defined (empirical) technique. Consequently, the method must be followed carefully, and must be performed consistently from day to day…